mouse elisa kits  (R&D Systems)

Journal: Frontiers in Pharmacology

Article Title: Dimethyl malonate suppresses succinate dehydrogenase to alleviate ischemia/reperfusion-induced acute lung injury

doi: 10.3389/fphar.2026.1746514

Figure Lengend Snippet: DMM reduced the IR-induced elevation of proinflammatory cytokine levels in BALF and SDH activity in lung tissue. (A) CINC-1 (B) IL-6, and (C) TNF-α levels in the BALF were measured using ELISA kits. In the IR group, levels of these cytokines were significantly higher compared to the control group. Treatment with 8 mM DMM markedly suppressed these increases following IR (D) SDH enzymatic activity was measured in lung tissue homogenates to evaluate target engagement. DMM significantly inhibited the IR-induced elevation of SDH activity. However, the anti-inflammatory effects and the inhibition of SDH activity of DMM were reversed when co-administered with 8 mM DMS. The measurement data are shown as the means ± SDs (n = 6 for each group). ***p < 0.001 indicate statistical significance compared with the control group, +++ p < 0.001 indicates statistical significance compared with the IR group, and ###p < 0.001 indicates statistical significance compared with the IR + DMM group.

Article Snippet: We utilized commercially available mouse ELISA kits (R&D Systems Inc., Minneapolis, MN, USA) to assess the concentrations of CINC-1, IL-6 and TNF-α in the BALF.

Techniques: Activity Assay, Tissue, Enzyme-linked Immunosorbent Assay, Control, Drug discovery, Inhibition

Journal: Frontiers in Pharmacology

Article Title: Dimethyl malonate suppresses succinate dehydrogenase to alleviate ischemia/reperfusion-induced acute lung injury

doi: 10.3389/fphar.2026.1746514

Figure Lengend Snippet: DMM attenuated HR-induced SDH activity, proinflammatory cytokine production and NF-κB signaling in MLE-12 cells. (A) The levels of KC/CXCL-1 in the cell culture supernatant were measured using ELISA (B) SDH enzymatic activity was measured in MLE-12 cell lysates to evaluate target engagement. DMM significantly inhibited the HR-induced elevation of SDH activity, which was reversed by DMS supplementation (C,D) Western blot analysis was used to evaluate p-IκB-α and IκB-α expression and p-NF-κB levels in MLE-12 cells subjected to 2 h of hypoxia followed by 1 h of reoxygenation. The measurement data are shown as the means ± SDs (n = 3 for each group). **p < 0.01 and ***p < 0.001 indicate statistical significance compared with the control group; + p < 0.05, and +++ p < 0.001 indicate statistical significance compared with the HR group; and ##p < 0.01 and ###p < 0.001 indicate statistical significance compared with the HR + DMM group.

Article Snippet: We utilized commercially available mouse ELISA kits (R&D Systems Inc., Minneapolis, MN, USA) to assess the concentrations of CINC-1, IL-6 and TNF-α in the BALF.

Techniques: Activity Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Drug discovery, Western Blot, Analysis, Expressing, Control

Journal: Porcine Health Management

Article Title: Parabacteroides distasonis ameliorates ETEC-induced diarrhea in weaned piglets by regulating intestinal barrier function and intestinal inflammation

doi: 10.1186/s40813-026-00525-1

Figure Lengend Snippet: Effects of P. distasonis gavage on intestinal cytokine levels in weaned piglets after ETEC challenge. ( A ) Relative mRNA expression levels of inflammatory cytokines ( TNF-α , IL-1β , IL-6 , IL-8 ) and anti-inflammatory cytokine ( IL-10 ) as well as interferon-γ ( IFN-γ ) in the ileum. ( B ) Concentrations of TNF-α, IL-1β, IL-6, IL-8, IL-10, and IFN-γ in the ileal mucosa. Data are presented as the Mean ± SME. “*”indicated 0.01 ≤ P ≤ 0.05, “**” indicated 0.001 ≤ P ≤ 0.01, and “***” indicated P ≤ 0.001

Article Snippet: For the quantification of cytokine levels in ileal mucosa, a commercial porcine cytokine multiplex immunoassay kit (RayBiotech, Norcross, GA, USA) was used, and cytokine concentrations were calculated using QAP-CYT-1-SW software (RayBiotech, Peachtree Corners, GA, USA).

Techniques: Expressing

Journal: medRxiv

Article Title: Trajectories of brain structure and function in young adult carriers of genetic frontotemporal dementia variants

doi: 10.64898/2026.06.08.26355165

Figure Lengend Snippet: No differences were observed in plasma NfL or GFAP across ages 18 to 30 between variant carriers and non-carriers of C9orf72, GRN, or MAPT .

Article Snippet: In ALLFTD, plasma NfL concentrations were measured using the Simoa NF-Light digital immunoassay (Quanterix, Cat. No. 103186), and plasma GFAP using the Simoa GFAP digital immunoassay (Quanterix, Cat. No. 102336, Lot 503909), both on the HD-X Analyzer.

Techniques: Clinical Proteomics, Variant Assay